image-based capillary electrophoresis services
Isoelectric focusing (icIEF) is a powerful technique for separating proteins based on their isoelectric point (pI), revealing subtle charge differences critical for biopharmaceutical characterization. Agile BioScience offers advanced icIEF analysis to support your product development and quality control.
Why icIEF Matters
- Charge Variant Analysis: Identifies post-translational modifications, C-terminal lysine variation, glycosylation patterns, and other changes that alter protein charge.
- Manufacturing Consistency: Ensures batch-to-batch reproducibility and safeguards product quality.
- Isoform Separation: Enables isolation and characterization of distinct isoforms for in-depth analysis.
- Regulatory Compliance: Supports thorough characterization aligned with ICH guidances.
Our Approach:
- High-Resolution Separation: Our icIEF systems provide excellent resolution, detecting even minor charge differences.
- Isoform Fraction Collection: We couple icIEF with LC-MS/chromatography for targeted analysis of specific isoforms.
- Expertise in Interpretation: Our team translates icIEF data into actionable insights about your protein’s charge profile.
Benefits of Choosing iCE Analysis Services of Agile BioScience:
- Comprehensive Characterization Gain a detailed understanding of your protein’s charge variants.
- Troubleshooting Support: Identify the root cause of charge heterogeneity and optimize your process.
- Confident Decision-Making: Make informed decisions based on our precise icIEF analysis.
Contact us to learn how icIEF can empower your biopharmaceutical development.
Characterization
- Dynamic Light Scattering (DLS)
- Micro Flow Imaging (MFI)
- Liquid Chromatography
- Circular Dichroism (CD)
- SEC-MALS
- SV-AUC
- Fourier-Transform Infrared (FTIR)
- Differential Scanning Calorimetry (DSC)
- Secondary, Tertiary and Higher Order Protein Structure
- CE-SDS
- Intact Molecular Weight Analysis
- Oligosaccharide Profiling Services
- Disulfide Bond Mapping Services
- N- and O-linked glycosylation services
- Peptide Mapping Services
- C-terminal Sequencing
- N-Terminal Sequencing
- Amino Acid Analysis
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